Evolutionary genetics of sex differences, sexually antagonistic genetic variation, experimental evolution

Female-limited X-chromosome evolution

Female Drosophila melanogaster

Female Drosophila melanogaster.

This project is a follow-up to a previous investigation of male-limited X-chromosome (MLX) evolution in Drosophila melanogaster. By using an FM balancer chromosome, we have forced the X to only be expressed in females for many generations. This results in a relaxation of counter-selection in males, and causes the X to evolve to maximize female fitness. The female-limited X-chromosome evolution (FLX) experiment will therefore help to resolve a number of outstanding questions, for example whether the magnitude of the response is the same in FLX evolution as in MLX evolution, and whether it is the same traits which respond. Males are generally expected to be subject to stronger sexual selection than females, so it is possible that both sexes are displaced towards the male optimum for such traits. In that case we would expect to see a larger change as result of FLX evolution in traits that are sexually selected in males (but not male-limited in expression) relative to traits that are subject to natural selection in either sex. Another exciting question which the FLX experiment may be able to shed some light on is the importance and nature of dominance and epistasis in Drosophila. By expressing evolved X-chromosomes in females in a heterozygous (i.e. one FLX-evolved X-chromosome and one Control X-chromosome) or homozygous (i.e. two FLX-evolved X-chromosomes) state, we can test for fitness-related dominance and epistatic variance on the X-chromosome. Theory suggests that dominance should be important for X-linked sexually antagonistic genetic variation, and results from the MLX experiment suggest that epistatic interactions between the X-chromosome and the autosomes are common for sexually antagonistic loci.

We have confirmed that FLX females have indeed responded to the selection imposed, by increasing both fecundity and body size. Males have increased in body size as well, and there is evidence that this change is partly due to coevolution between the X-chromosome and the autosomes. We are currently investigating the genomic basis of the response to the selection treatment.

Students: Katrine Lund-Hansen, Yesbol Manat

Collaborators: Ted Morrow, University of Sussex